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. 2019 Dec 16;9:19190. doi: 10.1038/s41598-019-55747-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of Aubergine with symmetrically dimethylated arginines in Sf9 cells. (A) Aubergine was co-expressed with sDMA-generating methyltransferase Dart5 and methylosome protein Valois in Sf9 cells. (B) Expression of aub/dart5-vls bacmids using different multiplicity of infection (MOI) ratios resulted in the generation of sDMAs in Aub purified from Sf9 cells reaching the saturation levels at the MOI ratio of 5:5 for both bacmids. sDMAs in Aub were detected by Western blot with SYM11 antibody (bottom panel). Aub was detected with anti‐c‐Myc antibody (top panel). Negative controls (just Sf9 cells extracts and Aub from Sf9 cells expressing aub bacmid without dart5-vls bacmid) are shown in the first two lanes.