Skip to main content
. 2019 Dec 16;9:19190. doi: 10.1038/s41598-019-55747-x
Nuclease solution 0.1 M MgCl2, 5U universal nuclease (Thermo Fisher Scientific) in PBS
IP wash buffer 0.1% Tween-20, 1% IGEPAL CA-630, 0.1 M Glycine, 1 M NaCl in PBS
ATP buffer 5 mM ATP, 15 mM MgCl2, protease inhibitors, 1 mM phenylmethanesulfonyl fluoride (PMSF) in IP Wash Buffer
c-Myc peptide elution buffer 0.1 mg/ml c-Myc peptide, 300 mM Imidazole in PBS
Aub Binding Buffer (ABB) 1 M NaCl, 50 mM NaH2PO4, 10 mM Imidazole, pH 8
Arginine Wash Buffer (AWB) 500 mM NaCl, 50 mM NaH2PO4, 40 mM Imidazole, 250 mM Arginine, pH 8
Non-Arginine Wash Buffer (NonWB) 500 mM NaCl, 50 mM NaH2PO4, 40 mM Imidazole, pH 8
Aub Elution Buffer (AEB) 500 mM NaCl, 50 mM NaH2PO4, 300 mM Imidazole, pH 8
Tris-buffered saline (TBS) 25 mM Tris/Tris-HCl, 0.13 M NaCl, 2.7 mM KCl