Nuclease solution |
0.1 M MgCl2, 5U universal nuclease (Thermo Fisher Scientific) in PBS |
IP wash buffer |
0.1% Tween-20, 1% IGEPAL CA-630, 0.1 M Glycine, 1 M NaCl in PBS |
ATP buffer |
5 mM ATP, 15 mM MgCl2, protease inhibitors, 1 mM phenylmethanesulfonyl fluoride (PMSF) in IP Wash Buffer |
c-Myc peptide elution buffer |
0.1 mg/ml c-Myc peptide, 300 mM Imidazole in PBS |
Aub Binding Buffer (ABB) |
1 M NaCl, 50 mM NaH2PO4, 10 mM Imidazole, pH 8 |
Arginine Wash Buffer (AWB) |
500 mM NaCl, 50 mM NaH2PO4, 40 mM Imidazole, 250 mM Arginine, pH 8 |
Non-Arginine Wash Buffer (NonWB) |
500 mM NaCl, 50 mM NaH2PO4, 40 mM Imidazole, pH 8 |
Aub Elution Buffer (AEB) |
500 mM NaCl, 50 mM NaH2PO4, 300 mM Imidazole, pH 8 |
Tris-buffered saline (TBS) |
25 mM Tris/Tris-HCl, 0.13 M NaCl, 2.7 mM KCl |