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. 2019 Dec 16;9:19153. doi: 10.1038/s41598-019-55579-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In order to provide ultra-high-resolution (100-point) DEP spectra, K452 cells were analysed by 20-point spectra (top row) that, when combined, provided a single spectrum with 100 equally-spaced points (shown here as Combined run 1). Three more 100-point spectra (Combined runs 2–4) were performed separately; these were then combined into a single 400-point spectrum (main panel). Best-fit models were generated for the 400-point spectrum (shown) and compared to best-fit models for 100-point and 20-point spectra in order to determine whether 20 points is as accurate as higher-resolution spectra for robust data extraction.