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. 2019 Dec 16;9:19161. doi: 10.1038/s41598-019-55605-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of C-PC treatment effect on the COX-2 expression in the CT-26 cells. (A) The relative levels of COX-2 mRNA at the CT-26 cells after treatment with different concentrations of C-PC (0, 50, 100, 200, and 300 µg/mL) according to qRT-PCR analyses. (B) western blot analyses of beta-actin and (C) COX-2 proteins expression after treatment with different concentrations of C-PC (0, 50, 100, 200, and 300 µg/mL). The 1, 2, 3, 4, 5, and 6 lanes represent the protein marker, 0, 50, 100, 200, and 300 µg/mL samples, respectively. The figure displays the full-length blots with no cropping. (D) The normalized COX-2 protein levels at different treatment groups (0, 50, 100, 200, and 300 C-PC µg/mL). (E) The PGE2 level at the CT-26 cells’ culture media after 24 h incubation with different concentrations of C-PC (0, 50, 100, 200, and 300 µg/mL). (*P < 0.05, ns: not significant).