Figure 11.

(A) Cell viability relative to the unlabeled control MSC was determined 12 h after exposure to Fe₃O₄@Al(OH)₃ NPs post-labeling: A significant reduction in the cell survival was seen for concentrations > 100 μg NP mL⁻¹. (B) Population doubling times (PDT) were determined after exposure of MSC to NPs for 12 h and subsequent incubation in NP-free medium for two days (solid bares) and six days (striped bares). Significant differences with respect to PDTs were only seen for concentrations of 250 μg NP mL⁻¹ at two days after the removal of NPs from the medium. Three replicates were measured per condition. Differences relative to unlabeled cells were assessed based on a one-way ANOVA with Bonferroni correction. * p < 0.05 and *** p < 0.001.