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. 2019 Dec 16;10:5713. doi: 10.1038/s41467-019-13360-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Polysome profiles of persister (Per) vs. parental (Par) cells in the presence or absence of silvestrol (silv, 30 nM for 4h). b, c Total mRNAs and polysome-bound mRNAs were purified for m⁶A dot blot analysis (b) and the semi-quantification of the dot blot intensity (c). Polysome-derived mRNA m⁶A intensity was normalized to the total lysate (input)-derived mRNA m⁶A. n = 3, unpaired t-test. d RT-qPCR quantification of m⁶A enrichment in the translationally upregulated mRNA in persister cells upon the indicated treatment (n = 3, **p-value < 0.01, unpaired t-test). The raw data of c and d are available in Source Data.