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. 2019 Dec 5;13(6):1126–1141. doi: 10.1016/j.stemcr.2019.11.003

Figure 4.

Figure 4

Generation of Kidney Organoids and T Lymphocytes

(A) Heatmaps indicating log2 fold change of marker gene expression normalized to iPSCs (left) or D7 (right) (independent experiments [PGPC3/17 = 1, PGPC14 ≥ 2]; technical replicates ≥3).

(B) Representative images of immunohistochemistry of kidney organoid sections labeled with anti-WT1 (Wilms tumor 1), anti-LTL (labeled with lectin), and anti-ECAD (E-cadherin) antibodies (independent experiments ≥3 for each line). Scale bars represent 100 μm. Color channels were independently altered to adjust contrast for publication.

(C) Average differentiation efficiency of iPSC lines after magnetic-activated cell sorting for CD34 (independent experiments = 4; SEM).

(D) T lymphocyte marker expression during T cell maturation from HSPCs.

(E) D8+14 cells were labeled with anti-CD34, anti-CD7, and anti-CD5 antibodies and measured with flow cytometry (independent experiments = 3).

(F) D8+42 cells were labeled with anti-CD34, anti-CD5, anti-CD7, anti-CD4, anti-CD8b, anti-CD3, anti-T cell receptor alpha/beta, and anti-T cell receptor gamma/delta then measured by flow cytometry (independent experiments = 3).