Figure 4.

Single-Cell Gene Expression Analysis

(A) Schematic depiction of single-cell gene expression analysis at 48-h and 14-day time points.

(B) Heatmap of expression levels of the panel of genes in individual cells after 48 h in E8, E6, or BA. Genes upregulated in BA cells are highlighted (red box).

(C) t-SNE plot of individual cells, with E8 cells in blue, E6 in green, and BA in red.

(D) Genes in the red box were analyzed for tissue enrichment using EnrichR.

(E) Heatmap of expression levels of the panel of genes in individual cells after 14 days. Genes upregulated in BA cells are highlighted in red boxes and genes upregulated in E8 and E6 cells are highlighted in the blue box.

(F) t-SNE plot of individual after 14 days (E8 blue, E6 green, and BA red).

(G) Genes upregulated in BA cells (red boxes in E) were analyzed for tissue enrichment in EnrichR (top, red bars), as were genes upregulated in E8/E6 cells (blue box in E), bottom graph with blue bars.

See also Figure S2.