Figure 6.

Pretreatment with BA for 48 h Repressed Neuroectoderm Potential

(A) Schematic depicting the neuro-induction protocol using E8-, E6-, or BA-pretreated cells as the input population.

(B and C) Quantification (B) and representative images (C) of immunofluorescent staining for SOX2 and TBXT after 48 h, and SOX2 and Nestin after 5 days.

(D) Gene expression analysis after 48 h, 5 days in E6, and 5 days in dual SMAD inhibition (DI) medium for E8-, E6-, and BA-pretreated cells. Pluripotency (OCT4, NANOG, SOX2), neuroectodoerm (SOX2, PAX6, NES, OTX2), and mesoderm/endoderm (AFP, KDR, MIXL1) genes were assessed.

Error bars represent SEM, n = 3 independent experiments, ^∗p < 0.05, ^∗∗p < 0.01, ‡ = p < 0.001 for (B) and (D). Scale bar, 200 μm.