Figure 2.

ESAM Deficiency Was Associated with High Mortality in Fetuses after E15.5

(A) Phenotypic comparison of ESAM Homo fetuses with their littermates after E15.5. Typical images of E17 (upper) and E17.5 (lower) fetuses are shown. Living and dead ESAM Homo fetuses are surrounded by blue and red squares, respectively.

(B) Absolute number of Ter119^– CD71⁺ immature erythroblasts and Ter119⁺ mature erythroblasts/erythrocytes in FLs at E14.5 (n = 3, each group).

(C) Representative smear specimens of FLs at E14.5 (left). Arrows indicate orthochromatic erythroblasts, and arrowheads indicate enucleated erythrocytes. The percentages of orthochromatic erythroblasts per orthochromatic erythroblasts plus enucleated erythrocytes are shown (right) (n = 3, each group).

(D) Results of real-time qPCR analyses of E16.5 WT and ESAM Homo KO FLs (three independent experiments).

Data are shown as means ± SEMs. Statistically significant differences are represented by asterisks: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.