Figure 4.

ESAM-Null FL HSCs Caused an Anemic Phenotype after In Vivo Transplantation

Four hundred E14.5 FL LSK CD48^- cells from WT or ESAM Homo KO mice were transplanted into lethally irradiated CD45.1 recipient mice (WT, n = 4; Homo, n = 6). Fifteen weeks after transplantation, recipient mice were sacrificed and analyzed.

(A) Schematic showing the transplantation protocol.

(B) The percentages of CD45.1^– CD45.2⁺ donor-type chimerism in whole peripheral blood (PB, left) or bone marrow (BM, middle). Percentages of donor-type chimerism in Mac1⁺Gr1⁺ myeloid, B220⁺ B cell, and CD3⁺ T cell fractions (right).

(C) The number of LSK CD150⁺ CD48^– HSCs, Lin⁻ c-Kit⁺ Sca-1^– IL-7Ra⁻ CD4^– CD8^– IgM⁻ CD34⁺ FCγR^low common myeloid progenitors (CMPs), LSK Flt3⁺ IL-7Ra⁻ lymphoid-primed multipotent progenitors (LMPPs), and Lin⁻ c-Kit^low Sca-1^–/low Flt3⁺ IL-7Ra⁺ common lymphoid progenitors (CLPs) in BM.

(D) Peripheral blood cell counts of red blood cells (RBCs) and hemoglobin (Hb).

Data are shown as means ± SEM. Statistically significant differences are represented by asterisks: ^∗p < 0.05. See also Figure S2.