Table 4.
The effects of As2O3 on the differentiation of CD4+T cells under special polarizing cytokines. Th17 cells: Anti-CD3 (2 μg/mL), Anti-CD28 (4 μg/mL), IL-1β (10 ng/mL), IL-6 (20 ng/mL), IL-23 (100 ng/mL), TGF-β (1 ng/mL). iTreg cells: Anti-CD3 (2 μg/mL), Anti-CD28 (4 μg/mL), IL-2 (20 U/mL), TGF-β1 (2 ng/mL).
| As2O3 (μM) | Th17 cells (%) | STAT3 gene expression | Treg cells (%) | Foxp3 gene expression |
|---|---|---|---|---|
| veh | 0.2 | 0.9 | 4.2 | 1.2 |
| 0.5 | 1.5 | 2.5 | 1.5 | |
| 0.8 | 1.0 | 3.5 | 1.0 | |
| 1.2 | 0.6 | 5.0 | 2.0 | |
| 0.1 | 0.8 | 3.0 | 1.2 | |
| 0.3 | 1.8 | 4.0 | 0.5 | |
| 0 | 4.5 | 2.2 | 7.5 | 1.5 |
| 4.3 | 2.5 | 6.4 | 2.0 | |
| 3.5 | 2.1 | 8.5 | 2.4 | |
| 5.8 | 2.5 | 6.5 | 2.5 | |
| 13 | 2.0 | 7.5 | 1.5 | |
| 5.0 | 1.8 | 8.0 | 1.4 | |
| 0.5 | 23 | 2.0 | 9.0 | 1.8 |
| 4.0 | 2.4 | 9.8 | 2.3 | |
| 2.8 | 1.5 | 7.0 | 2.5 | |
| 3.0 | 1.0 | 8.0 | 3.5 | |
| 1.5 | 12 | 8.5 | 3.0 | |
| 2.0 | 0.8 | 9.5 | 2.5 |