TABLE 2.
Preselected genes with significant differences (uncorrected Poverall < 0.05) in expression levels1
| Gene symbol | Mean FC2 | FC differences | P value | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Address ID | ICR | CCR | Control | ICR-CCR | ICR-Control | CCR-Control | Uncorrected3 | Corrected4 | ||
| 7330035 | SFRP4 | secreted frizzled-related protein 4 | −0.13 ± 0.03 | −0.05 ± 0.03 | 0.01 ± 0.03 | −0.08 ± 0.04 | −0.15 ± 0.05 | −0.06 ± 0.04 | 0.003 | 0.24 |
| 6450672 | VEGFA | vascular endothelial growth factor A | 0.01 ± 0.01 | −0.05 ± 0.01 | 0.01 ± 0.02 | 0.06 ± 0.02 | 0.00 ± 0.02 | −0.06 ± 0.02 | 0.01 | 1 |
| 3940017 | LOX | lysyl oxidase | −0.23 ± 0.05 | −0.08 ± 0.05 | −0.04 ± 0.05 | −0.15 ± 0.07 | −0.19 ± 0.07 | −0.04 ± 0.07 | 0.02 | 1 |
| 3610129 | HSD17B1 | hydroxysteroid(17-β)dehydrogenase1 | −0.09 ± 0.02 | 0.02 ± 0.03 | −0.06 ± 0.03 | −0.10 ± 0.03 | −0.03 ± 0.04 | 0.07 ± 0.04 | 0.02 | 1 |
| 1980309 | IL8 | interleukin 8 | 0.12 ± 0.16 | −0.49 ± 0.16 | 0.03 ± 0.18 | 0.61 ± 0.23 | 0.09 ± 0.24 | −0.51 ± 0.24 | 0.02 | 1 |
| 2640543 | SFRP4 | secreted frizzled-related protein 4 | −0.22 ± 0.07 | −0.10 ± 0.06 | 0.02 ± 0.07 | −0.12 ± 0.09 | −0.24 ± 0.10 | −0.12 ± 0.09 | 0.03 | 1 |
| 4050022 | TSHR | thyroid stimulating hormone receptor | 0.03 ± 0.02 | −0.03 ± 0.02 | −0.01 ± 0.01 | 0.06 ± 0.02 | 0.04 ± 0.02 | −0.03 ± 0.02 | 0.03 | 1 |
| 2900129 | FTO | fat mass and obesity associated | −0.05 ± 0.03 | 0.05 ± 0.02 | 0.00 ± 0.02 | −0.10 ± 0.04 | −0.04 ± 0.04 | 0.05 ± 0.03 | 0.03 | 1 |
| 2680056 | CES1 | carboxylesterase 1 | −0.43 ± 0.07 | −0.19 ± 0.08 | −0.32 ± 0.07 | −0.25 ± 0.10 | −0.12 ± 0.10 | 0.13 ± 0.10 | 0.03 | 1 |
| 7330544 | ALDOC | aldolase C, fructose-bisphosphate | −0.40 ± 0.07 | −0.31 ± 0.09 | −0.16 ± 0.06 | −0.09 ± 0.11 | −0.24 ± 0.09 | −0.15 ± 0.11 | 0.04 | 1 |
| 3710523 | CYP19A1 | cytochrome P450, family 19, subfamily A, polypeptide 1 | −0.05 ± 0.02 | −0.01 ± 0.02 | 0.02 ± 0.02 | −0.04 ± 0.03 | −0.07 ± 0.03 | −0.03 ± 0.03 | 0.04 | 1 |
| 1570553 | IL8 | interleukin 8 | −0.02 ± 0.08 | −0.25 ± 0.08 | 0.00 ± 0.08 | 0.23 ± 0.11 | −0.01 ± 0.12 | −0.24 ± 0.11 | 0.04 | 1 |
| 840309 | LEP | leptin | −0.23 ± 0.06 | −0.05 ± 0.05 | −0.14 ± 0.04 | −0.18 ± 0.07 | −0.09 ± 0.07 | 0.08 ± 0.06 | 0.04 | 1 |
1Data were included from 150 participants; statistical analyses were performed with the use of an intention-to-treat approach. CCR, continuous calorie restriction; FC, fold change; ICR, intermittent calorie restriction.
2Mean log FCs ± SEs within groups were calculated as differences between the expression levels at baseline (T0) and the expression levels at week 12 (T1): log2(FC) = log2(T1/T0).
3 Pvalues for time-by-treatment interactions across all 3 study groups were calculated with linear mixed models adjusted for age and sex. No adjustment was made for multiple comparisons. P values for post hoc pairwise comparisons between individual study groups are shown in Supplemental Table 2.
4 P values for time-by-treatment interactions across all 3 study groups were calculated with linear mixed models adjusted for age and sex including Bonferroni correction for multiple comparisons (P values were multiplied by the number of genes, i.e., by 82).