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. 2019 Nov 7;13(6):1083–1098. doi: 10.1016/j.stemcr.2019.10.009

Figure 3.

Figure 3

Expansion and Characterization of Naive iPSCs

(A) Morphology of naive iPSC culture on MEFs at passage 1 after reprogramming.

(B) Flow cytometry analysis of SUSD2 and CD24 expression in HDF16-, HDF75-, and BJ-derived naive iPSC cultures at passage 2, compared to chemically reset H9 naive cells.

(C) SUSD staining of naive iPSC cultures of indicated origin after sorting and subsequent passaging (P).

(D) Immunostaining for naive markers in expanded naive iPSCs (BJ derived).

(E) qRT-PCR analysis of marker expression in expanded naive iPSCs of indicated origins and embryo-derived naive HNES1 cells. Data are normalized to expression in conventional H9 cells. Error bars indicate SD of two technical replicates.

Scale bars, 100 μm. See also Figure S3.