Figure 4.

Expansion of Naive iPSCs from Single Colonies

(A) qRT-PCR analysis of pluripotency markers in six expanded naive iPSC colonies at indicated passages. Two isogenic conventional iPSC colonies (piPSC1 and piPSC2) expanded in parallel and embryo-derived HNES5 cells are included for comparison. Error bars indicate SD of two technical replicates.

(B) DNA content analysis from flow cytometry profiles of cells stained with propidium iodide. Diploid genome population is labeled as 2N, 4N indicates cells in G2 and/or tetraploid, hyperpolypoid is >4N.

(C) Chromosome analyses of expanded niPSC colonies at indicated passasages (P).