Figure 5.

Differentiation of Capacitated Naive iPSCs

(A) Flow cytometry analysis of SOX17 and CXCR4 expression after 3 days definitive endoderm induction of primed S6EOS and capacitated niPSC4 cells.

(B) Immunostaining for FOXA2 and SOX17 after 3 days definitive endoderm induction of niPSC2.

(C) qRT-PCR analysis of definitive endoderm markers after 3 days induction of niPSC2.

(D) Immunostaining for SOX1 and PAX6 after 10 days neuroectoderm induction of niPSC2.

(E) qRT-PCR analysis of neuroectoderm marker expression.

(F) Immunostaining for TBX6 after 6 days of paraxial mesoderm induction of niPSC2.

(G) qRT-PCR analysis of paraxial mesoderm markers.

Scale bars, 100 μm. Error bars indicate SD of three technical replicates.

See also Figure S4.