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. 2019 Nov 7;13(6):1099–1110. doi: 10.1016/j.stemcr.2019.10.007

Figure 1.

Figure 1

Induction of a Hematoendothelial Program Following Transfection hiPSCs with ETV2 mmRNA

(A) Schematic diagram of optimized protocol used for generation of neutrophils from hiPSCs in serum-, xeno-, and feeder-free conditions.

(B) Representative light microscopy images showing hematoendothelial development following transfection IISH2i-BM9 hiPSCs with ETV2 mmRNA and culture with FGF2 and GM-CSF. Scale bars, 300 μm.

(C and D) Representative experiment showing kinetics of CD144 and CD43 expression (percentages (C) and flow cytometry dot plots (D) in cultures.

(E) Expression of CD144, CD43, and CD45 on day 6, 8, and 9 after ETV2 transfection. Bars show mean ± SE for 3 independent experiments.

(F) Yield of floating myeloid cells in step 2 cultures from 106 undifferentiated hiPSCs. Bars show mean ± SE for 2 independent experiments.

See also Figure S1.