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. 2019 Nov 7;13(6):1099–1110. doi: 10.1016/j.stemcr.2019.10.007

Figure 2.

Figure 2

Formation of Myeloid Progenitors in hiPSC Cultures Transfected with ETV2 mmRNA

(A) Flow cytometric analysis of CD34, CD33, and CD235a/CD41a expression in ETV2 mmRNA-transfected IISH2i-BM9 cultures differentiated in the presence of GM-CSF and FGF2.

(B) Representative experiment shows kinetics of CD34 and CD33 expression.

(C) Bar graphs show proportion of CD34+ and CD34− cells in floating fraction at indicated days of differentiation. Results are mean + SE for 3 experiments.

(D) Kinetics of CD11b and CD16 expression in cells collected from step 2 cultures. Error bars are + SE for 3 experiments.

(E) Cytopsin showing the morphology of the myeloid progenitors generated in cultures after 10 days of differentiation. Scale bar, 20 μm.

(F) Colony-forming potential of myeloid progenitors formed in cultures. Bars show mean ± SE for 2 independent experiments performed in duplicates. Representative images of colonies formed by cells collected from step 2 cultures are shown. Scale bars, 790 μm.

(G) Yield of floating cells from IISH2i-BM9 hiPSCs in the presence of different cytokine combinations and UM171. Bars show mean ± SE for 3 (GM-CSF, FGF, and UM171) or 2 (remaining combinations) independent experiments.

(H) Effect of different cytokine combinations on phenotype of myeloid cells. GM is GM-CSF, FGF is FGF2, FLT is FLT3L, U is UM171. Error bars are ± SE for 3 experiments.