Figure 2.

Differentiation of Grafted Human iNPCs in Hippocampus of Immunodeficient Mice

(A) Total numbers of KU80⁺ human cells in the hippocampus of immunodeficient mice 2, 4, 6, and 12 months post transplantation (M p.t.).

(B) The coronal brain section harboring grafted GFP⁺ human iNPCs at 2, 4, 6, and 12 M p.t. (Top) Immunofluorescence analysis of KU80 expression among GFP⁺ cells (second panel). Cell nuclei were counterstained with DAPI.

(C) Immunofluorescence analysis of NEUN and GFAP expression in KU80⁺ cells. The dashed line indicates the KU80⁻NEUN⁺ host neurons mixing with grafted cells.

(D) Percentages of NEUN⁺ mature neurons and GFAP⁺ astrocytes among KU80⁺ grafted human cells shown in (C).

(E) Immunofluorescence analysis of subtypes of human iNPC-derived neurons among KU80⁺ cells at 4 M p.t.

(F) Quantification of the results shown in (E).

(G) Percentages of Ki67⁺ cells among KU80⁺ grafted cells at 1, 2, 4, 6, and 12 M p.t. shown in Figure S3D.

Scale bars, 500 μm (B), 50 μm (magnified image in B) and 25 μm (C and E). n = 3 mice per time point. Data are represented as scatterplots with mean ± SD. Related to Figure S3.