Figure 4.

The Functional Integration of Human iNPC-Derived Neurons into Local Synaptic Networks of the Host Hippocampus

(A) Representative traces from human neuronal cells on acute slices from mice brains 2, 4, and 6 M p.t. showing sEPSCs at a holding potential of −70 mV and sIPSCs at 0 mV. Single synaptic events were shown for comparison.

(B) The kinetics of sEPSC and sIPSC from human neuronal cells at 2, 4, and 6 M p.t. Data are represented as scatterplots with mean ± SD. One-way ANOVA followed by Tukey's post-hoc test. ^∗p < 0.05.

(C) The percentage of grafted human neuronal cells exhibiting sEPSCs and sIPSCs. Numbers of recorded cells (N) were shown on the graphs.

(D) Left, schematic drawing of the optogenetic experiment on acute slices. Right, blue light activated grafted human cells with ChR2 expression in voltage-clamp and current-clamp mode.

(E) Left, firing pattern of a neighbor host granular cell (GC). Right, light stimulation induced an increase in synaptic inputs in GC recorded in voltage-clamp mode at a holding potential of −70 mV. Sample size: 3 mice for 2 M p.t. and 6 M p.t. group, respectively; 6 mice for 4 M p.t. group; 3 mice for optogenetic assay.