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. 2019 Nov 12;24(12):827–835. doi: 10.1111/gtc.12730

Table 1.

Theoretical number of complementary regions of ASOs in the total size of the human pre‐mRNA sequences

Length of ASO (mer) Number of mismatches
0 1 2 3 4 5
27 <10−1 <10−1 <10−1 <10−1 <10−1 1.3
26 <10−1 <10−1 <10−1 <10−1 0.31 4.1
25 <10−1 <10−1 <10−1 <10−1 1.1 13
24 <10−1 <10−1 <10−1 0.23 3.6 43
23 <10−1 <10−1 <10−1 0.79 12 136
22 <10−1 <10−1 0.14 2.8 39 425
21 <10−1 <10−1 0.50 9.5 129 1,314
20 <10−1 <10−1 1.8 33 417 4,005
19 <10−1 0.24 6.5 111 1,335 >104
18 <10−1 0.92 23 375 4,216 >104
17 <10−1 3.5 83 1,249 >104 >104
16 0.27 13 294 4,115 >104 >104
15 1.1 49 1,029 >104 >104 >104
14 4.4 183 3,566 >104 >104 >104
13 17 679 >104 >104 >104 >104
12 70 2,508 >104 >104 >104 >104
11 279 9,196 >104 >104 >104 >104
10 1,114 >104 >104 >104 >104 >104