Fig. 2.

Silencing of EZH2 suppresses cell proliferation, invasion, and migration in glioma. a EZH2 mRNA level determined in glioma cells treated with oe-EZH2 or sh-EZH2 using RT-qPCR. b EZH2 protein band patterns detected in glioma cells treated with oe-EZH2 or sh-EZH2. c EZH2 protein level measured in glioma cells treated with oe-EZH2 or sh-EZH2 using Western blot analysis. d Proliferation of glioma cells treated with oe-EZH2 or sh-EZH2 detected using EdU assay (200×). e Migration of glioma cells treated with oe-EZH2 or sh-EZH2 detected using Transwell assay (200×). f Invasion of glioma cells treated with oe-EZH2 or sh-EZH2 detected using Transwell assay (200×); ^#p < 0.05 vs. glioma cells treated with oe-NC; ^&p < 0.05 vs. glioma cells treated with sh-NC; measurement data were shown as mean ± standard error; comparisons between two groups were conducted by unpaired t test. The experiment was repeated three times to obtain the mean value