Fig. 5.

MSCs transfer miR-133b to glioma cells through exosomes to repress the expression of EZH2. a Expression of miR-133b and EZH2 in MSCs co-cultured with glioma cells after restoration or depletion of miR-133b determine using RT-qPCR. b Internalization of exosomes by glioma cells observed under the inverted microscope (400×). c miR-133b and EZH2 expression in MSCs treated with GW4869 co-cultured glioma cells after restoration or depletion of miR-133b measured using RT-qPCR. d miR-133b expression in exosomes and MSCs after restoration or depletion of miR-133b measured using RT-qPCR. e miR-133b expression in glioma cells co-cultured with MSC-derived exosome by RT-qPCR; ^#p < 0.05 vs. MSCs co-cultured with glioma cells treated with mimic-NC; ^&p < 0.05 vs. MSCs co-cultured with glioma cells treated with inhibitor-NC; *p < 0.05 vs. glioma cells co-cultured with PBS; ^{^}p < 0.05 vs. glioma cells co-cultured with miR-133b mimic NC; ^@p < 0.05 vs. glioma cells co-cultured with miR-133b inhibitor NC; measurement data were shown as mean ± standard deviation; comparisons among multiple groups were assessed by one-way analysis of variance (Dunnett’s post hoc test). The experiment was repeated three times to obtain the mean value