. 2019 Dec 16;12:588. doi: 10.1186/s13071-019-3848-2

Table 1.

PCR oligonucleotide primers used for amplification of p104, Tp1 and Tp2 genes with their corresponding annealing temperatures and amplicon sizes

Gene locus	Primer name	Primer sequence (5′–3′)	Annealing temperature (°C)	Amplicon size (bp)	Reference
p104	IL3231 (Fw1)	ATTTAAGGAACCTGACGTGACTGC	60	496	[22]
	IL755 (Rev1)	TAAGATGCCGACTATTAATGACACC	60	496	[22]
	IL4243 (Fw2)	GGCCAAGGTCTCCTTCAGAATACG	55	277	[41]
	IL3232 (Rev2)	TGGGTGTGTTTCCTCGTCATCTGC	55	277	[41]
Tp1	Tp1-Fw1	ATGGCCACTTCAATTGCATTTGCC	50	432	[36]
	Tp1-Rev1	TTAAATGAAATATTTATGAGCTTC	50	432	[36]
	Tp1-Fw2	TGCATTTGCCGCTGATCCTGGATTCTG	55	405	[24, 37]
	Tp1-Rev2	TGAGCTTCGTATACACCCTCGTATTCG	55	405	[24, 37]
Tp2	Tp2-Fw1	ATGAAATTGGCCGCCAGATTA	50	525	[36]
	Tp2-Rev1	CTATGAAGTGCCGGAGGCTTC	50	525	[36]
	Tp2-Fw2	ATTAGCCTTTACTTTATTATTTWCATTYTAC	54	504	[24, 37]
	Tp2-Rev2	CTATGAAGTGCCGGAGGCTTCTCCT	54	504	[24, 37]

Abbreviations: Fw1, forward outer; Fw2, forward inner; Rev1, reverse outer; Rev2, reverse inner