Table 2.
Tp1 and Tp2 sequence diversity in cattle-derived T. parva from RDC and Burundi
| Gene locus | AEZ | Sample size | Nucleotide sequences | Amino acid sequences | |||
|---|---|---|---|---|---|---|---|
| Polymorphic sitesa | No. of allelesb | Nucleotide diversity ± SD | Polymorphic sitesa | No. of antigen variantsb | |||
| Tp1 | DRC AEZ1 | 31 | 12 + ind | 7 | 0.008 ± 0.002 | 7 + ind | 5 |
| DRC AEZ2 | 27 | 12 + ind | 8 | 0.007 ± 0.002 | 7 + ind | 6 | |
| DRC AEZ3 | 25 | 0 | 1 | 0 | 0 | 1 | |
| Burundi AEZ1 | 33 | 4 | 3 | 0.005 ± 0.0004 | 3 | 3 | |
| Overall Tp1 | 116 | 14 + ind | 11 | 0.005 ± 0.0007 | 7 + ind | 6 | |
| Tp2 | DRC AEZ1 | 25 | 166 | 7 | 0.13 ± 0.025 | 82 | 7 |
| DRC AEZ2 | 20 | 165 | 5 | 0.17 ± 0.016 | 81 | 5 | |
| DRC AEZ3 | 23 | 2 | 2 | 0.002 ± 0.0004 | 2 | 2 | |
| Burundi AEZ1 | 28 | 175 | 6 | 0.16 ± 0.017 | 85 | 5 | |
| Overall Tp2 | 96 | 181 | 10 | 0.14 ± 0.01 | 88 | 9 | |
aThe insertion region (ind) was excluded for the determination of the number of polymorphic sites and the nucleotide diversity
bAlleles represent distinct nucleotide sequences diverged at least by one substitution (Additional file 4: Figure S1, Additional file 8: Figure S2), while antigen variants represent predicted distinct protein sequences (Figs. 2, 4)
Abbreviations: AEZ, agro-ecological zone; ind, indels; SD, standard deviation