Table 3.
Tp1 and Tp2 CD8+ T cell epitope variants identified in cattle-derived T. parva from DRC and Burundi
| Tp1 epitope variants (Tp135–45) | Tp2 epitope variants | |||||
|---|---|---|---|---|---|---|
| Epitope 1 (Tp220–30) | Epitope 2 (Tp233–41) | Epitope 3 (Tp242–52) | Epitope 4 (Tp289–97) | Epitope 5 (Tp291–99) | Epitope 6 (Tp2131–140) | |
| VGYPKVKEEML (var-1, 32) | SHEELKKLGML (var-1) | DGFDRDALF (var-1, 2, 58) | KSSHGMGKVGK (var-1, 2, 58) | FAQSLVCVL (var-1, 2, 58) | QSLVCVLMK (var-1, 2, 58) | KTSIPNPCKW (var-1, 2, 58) |
| VGYPKVKEEII (var-3, 31, 34) | TEEELKKMGMV (var-55) | EGFDKEKLF (var-55) | KSSKSMGIVGR (var-55) | FVQSIMCVI (var-55) | QSIMCVINK (var-55) | VNDIPNPCKW (var-55) |
| VGYPKVKEEMI (var-33) | TEEELRKLGMV (var-56, 57, 59) | SNFDRESLF (var-56, 57, 59) | KSSHGMGKVGR (var-56, 57, 59) | FAQSILCVI (var-56, 57, 59) | QSILCVIKN (var-56, 57, 59) | ASDIPNPCKW (var-56, 57, 59) |
| SDNELDTLGLL (var-54) | PDLDKNRLF (var-53, 54) | LTSHGMGKIGR (var-54) | LAASIKCVS (var-54) | ASIKCVSHH (var-54) | KPSVPNPCDW (var-53, 54) | |
| SDDELDTLGML (var-53) | LTSHGMGRIGR (var-53) | FAASIKCVA (var-53) | ASIKCVAQY (var-53) | |||
| SDEELNKLGML (var-2) | ||||||
| SDEELNILGML (var-58) | ||||||
Notes: Epitope variants were identified using the reference amino acid positions presented in Additional file 2: Table S2. Numbers in brackets following the epitope sequences correspond to antigen variants carrying the epitopes (Figs. 2, 4). Epitope variants described for the first time are in italic and those found in the Muguga cocktail vaccine are in bold. Tp2 antigen variants var-1 and var-2 are found in Muguga (identical with Serengeti-transformed) and Kiambu-5 strains, respectively. Tp1 antigen variant var-1 is found in Muguga (identical with Serengeti-transformed and Kiambu-5)
Abbreviations: Var-1 to var-59, antigen variant names