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. 2019 Dec 16;16:158. doi: 10.1186/s12985-019-1268-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Electron-lucent viral factory and treatment with membrane trafficking inhibitor. a-b TEM image at 24 h.p.i shows that Orpheovirus present an electron-lucent VF (contoured in red and in detail), which occupy a large part of the host and is observed in perinuclear regions. We also observed membrane recruitment inside the VF (blue), and the host nucleus remains present during the infection. c Treatment with BFA impacts both the formation of VFs and morphogenesis of new particles. VF: viral factory; N: nucleus; Mitochondria are highlighted in purple; *viral particles