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. 2019 Dec 16;16:158. doi: 10.1186/s12985-019-1268-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Orpheovirus particles are released from the cell by exocytosis. a TEM image at 24 h.p.i evidencing new particles in peripheral regions in the host cell cytoplasm (white arrow). b and c Some particles were observed involved by one or more membranes (highlighted in yellow). d More than one particle in the same vacuole were evidenced. e IF assays at 12 h.p.i showing fusiform cells and an increase of particles outside the host cells released by exocytosis (white arrows). f Count of Orpheovirus particles in the supernatant over the replication cycle (M.O.I. = 5)