Table 3.

Top candidates selected from ReVac’s output for NTHi and M. catarrhalis (M.W/pI represent molecular weights and isoelectric points)

Example locus tag	Amino acid length	M.W./pI	Annotation	Gene
NTHi
84P48H1_01193	562	62.24/9.43	Hemopexin transporter	hxuB
84P8H1_00650	274	30.48/8.97	Lipoprotein E precursor	Hel (P4)
M. catarrhalis
ADC73_RS07905	405	44.01/9.47	Hypothetical protein (porin family)	None
E9Y_00353	537	60.40/8.95	Protein of unknown function (DUF560)	None
M137P16B1_1805	344	38.37/8.95	Gram-negative porin protein	None
AO373_1452a	331	36.16/9.9	Ferric iron ABC transporter iron-binding protein	None

All the above candidates were surface exposed, predicted antigenic, conserved core proteins with low autoimmunity and no repeat regions. Further information about these candidates are available in Additional files 2 and 3 respectively. ^aPresent in M. canis