FIG. 1.

Clustering of in vitro human differentiation data after batch correction. (A) Hierarchical clustering of samples at different stages of differentiation. The colors represent different sources of data. See Supplementary Table S1 for sample annotation. (B) NMF on human in vitro data after batch correction. All the samples clustered into three distinct groups of early, mid, and late stages of differentiated cells. (C) PCA. The samples aligned according to their differentiation status in the principal component space, starting with endodermal cells followed by HLCs, and the most mature cell types to the far right. The colors represent the different stages of the samples and the shapes represent the different sources of the data. HLCs, hepatocyte-like cells; NMF, non-negative matrix factorization; PCA, principal component analysis; PHHs, primary human hepatocytes. Color images available online at www.liebertpub.com/scd