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. 2019 Jul 17;25(13-14):1023–1036. doi: 10.1089/ten.tea.2018.0202

FIG. 4.

FIG. 4.

(a) Active tension produced by differentiated skeletal muscle rings stimulated electrically at 1 Hz (n = 7, 6, and 5). (b) Passive tension of differentiated skeletal muscle rings (n = 7, 6, and 5). (c) Active tension (solid line) and passive tension (dotted line) plotted with time for a single frozen undifferentiated biobot stimulated at 1 Hz. (d) Active tension of four frozen differentiated biobots recovered measured 24 and 48 h postthaw. (e) RT-qPCR fold change in mRNA expression of various myogenic markers in differentiated muscle, which had previously been frozen while undifferentiated. Expression is normalized to the unfrozen control (mean ± SD, n = 3). (f) RT-qPCR fold change in mRNA expression in muscle frozen differentiated (mean ± SD, n = 3). (*p < 0.05, **p < 0.01). RT-qPCR, quantitative reverse transcription PCR.