FIG. 4.

Transcriptomic analysis of male and female hESCs and progenitors of trophoblastic cells. (A) Cluster dendrogram of all RNA-Seq samples (females, pink; males, blue) based on Minkowski distance (ggplot). (B) PC analysis for all hESC (d0) and progenitors of trophoblastic cells (d5) samples, with female samples in pink/green and male samples in blue/purple. The percentage contribution of each principal component is displayed in each axis. (C) Log2 reads (CPM) for pluripotency markers among all samples. Two-tailed t-tests were performed to calculate significance between undifferentiated and trophoblastic cells (**P < 0.005; ****P < 0.0001). (D) Log2 reads (CPM) for markers of the early human trophoblast for male and female samples. Two-tailed t-tests were performed to calculate significance between undifferentiated and differentiation day 5 cells (**P < 0.005; ****P < 0.0001). RNA-Seq, RNA-sequencing. Color images available online at www.liebertpub.com/scd