FIG. 2.

Fluorescence microscopy analysis of EV uptake by cells at different temperatures. (A) Images of HEK293 cells incubated at different temperatures. (B) Quantification of minimal and maximal densities of HEK293 cells. Each point indicates one cell. (C) Images of NIH3T3 cells incubated at different temperatures. (D) Quantification of minimal and maximal densities of NIH3T3 cells. miR133a-3p-enriched EVs were labeled by PKH26 and visualized with excitation of 550 nm. The nuclei were stained by DAPI. The experiments were performed twice. In each experiment, cells on four coverslips (placed in 24-well plates) were treated under a condition. Shown are representative images. ***p < 0.0001 when compared with other groups (Tukey's multiple comparison test after ANOVA). ANOVA, analysis of variance.