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Competitive binding assay with human serum and monovalent Rha (○), tetravalent (▪, 3) and hexadecavalent (•, 6) conjugates. The assay measures the inhibition of the binding of human anti‐Rha IgM to Rha coated on microplate wells (PAA‐Rha). The binding was revealed with Alexa‐Fluor488‐labeled goat anti‐human IgM. Inhibition percentage versus the logarithm of the Rha conjugate concentration (in mm) is plotted. Data reported are an average of three independent experiments.
