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. 2019 Dec 17;16:266. doi: 10.1186/s12974-019-1632-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — A Iba1 IHC for CTRL (a–c), 1 h RS (d–f), and 2 h RS (g–i) after saline and α-blocker treatment, respectively. B Enlargement of the boxed areas in A. Scale bar: 50 μm in A and 20 μm in B. C Histogram demonstrating the cell size of Iba1-ir microglia in the HT at CTRL, 1 h RS, and 2 h RS following saline and α-blocker treatment. D Histogram showing the intersections count. E Histogram demonstrating the cell count of Iba1-ir microglial cells in the HT at CTRL, 1 h RS, and 2 h RS following saline and α-blocker treatment. The asterisks indicate a statistical difference between CTRL and stressed rats (^*p < 0.05, ^**p < 0.01, n = 4)