Fig. 2.

A, B Morphological changes of Iba1-ir (A) and GFAP-ir (B) cells following RS in the HT. The upper panels show low-power photomicrographs of Iba1 and GFAP immunoreactivity in the HT of the control (CTRL) (a), 1 h RS (b), and 2 h RS (c). Panels (d), (e), and (f) are enlargements of panels (a), (b), and (c), respectively. Panels (g), (h), and (i) are enlargements of boxed areas (d), (e), and (f), respectively. Arrows indicate resting (g) and activated (h, i) microglia. Scale bar: 200 μm at low-power, 20 μm at middle-power, and 10 μm at high-power magnification. C, D Histograms showing the quantification of the cell size of Iba1-ir microglia and GFAP-ir astrocytes in the HT, HC, and TM at CTRL, 1 h RS, 2 h RS, and 4 h RS, respectively. E Histograms showing the number of Iba1-ir microglia in the HT, HC, and TM at CTRL, 1 h RS, 2 h RS, and 4 h RS, respectively. The asterisks indicate a statistical difference between post-RS and control for each group. ^*p < 0.05, n = 4. Data are presented as means ± SEM. F No immunoreactivity to Ki67 was detected in the HT at 1h RS. Scale bar: 10 μm