Figure 7.

Effect of 5zox pretreatment for bone marrow‐derived mesenchymal stem cells (BMMSCs) on cell transplantation. (A): Duration of 5zox‐induced quiescence after stopping 5zox supplementation in the cultured BMMSCs. Red arrow heads show timing of medium change with 5zox‐fresh medium. (B): Detection of apoptosis/necrosis by PI/AnV staining. BMMSCs from EGFP mice were treated with 20 nM 5zox for 6 days, transplanted into femur muscles and collected at 48 hours after transplantation and analyzed for EGFP+ populations by FACS. (C): Analysis of intracellular ROS levels using the CellROX dye. Isolation of the transplanted cells was performed as above. (D): Remaining EGFP+ cell numbers in the recipient BMs. Asterisks represent significant differences (p < .05, n = 6) compared with the two groups. (E): Rate of undifferentiated BMMSC detected as PDGFRα/Sca1 double‐positive population (p < .05, n = 6).