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. 2019 Dec 10;10:2830. doi: 10.3389/fmicb.2019.02830

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Copyright © 2019 Bai, Lin, Li, Zhou, Liu, Zhong, Wu, Jiang, Du, Yang, Xie and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lyophilized CORDS assay. (A). Workflow for the lyophilized CORDS assay. (B) Verification of tubes B and C. (C) Accelerated stability test on tube B at 37°C. (D) Accelerated stability test on tube C at 4°C. (E) Reduction of the incubation time for LbCas12a cleavage. T and C in panels (C–E) represents the test and control bands, respectively.