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PCR to test the efficiency of polymerases in amplifying PCR products from supernatants from different spore concentrations of the A. fumigatus wild‐type strain with primers ITS1/D2 (expected PCR band sizes is ∼1.2 kb). (A) Phusion High‐Fidelity DNA polymerase (New England Biolabs). (B) MyTaq RED Mix DNA polymerase (Bioline). P: positive PCR control amplified from genomic DNA (50 ng) of the A. fumigatus wild‐type strain; N: negative control (no DNA).
