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. 2019 Aug 20;54(1):e89. doi: 10.1002/cpmc.89

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© 2019 The Authors. Current Protocols in Microbiology published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Spore PCR of the AYG1 gene and its flanking regions to test the range of PCR products effectively amplified using different concentrations of A. fumigatus wild‐type spores. The expected PCR band sizes are as follows: 622 bp (1), 1546 bp (2), 2241 bp (3), 3005 bp (4), 3744 bp (5), 4515 bp (6), and 5259 bp (7); P: positive control for the PCR reaction using genomic DNA (50 ng) of the A. fumigatus wild‐type strain as template, with the expected band size of 5259 bp; N: negative control (no DNA).