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. 2019 Oct 30;58(50):18252–18256. doi: 10.1002/anie.201911315

Figure 2.

Figure 2

Dysfunctional KR domain in the aur biosynthetic pathway leads to the production of 2. A) Non‐colinear polyketide biosynthesis in the aur pathway. Inactive ACP0 and AT4 are indicated with brackets. B) Model for lut biosynthesis. β‐Keto reduction does not take place in AurB, one module in AurC is skipped. C) S. albus::pHY127 (KR2 null mutant) shows strong yellow pigmentation on MS agar plate. The catalytic residue tyrosine was mutated to phenylalanine in YAAAN motif to create KR2 null mutant. D) HPLC profiles of (i) reference of 1, (ii) reference of 2, (iii) metabolites of heterologous expression strain S. albus::pHJ48 (S. albus_aur PKS), and (iv) KR2 null mutant, UV detection is at 350 nm.