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. 2019 Oct 30;58(50):18252–18256. doi: 10.1002/anie.201911315

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© 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Impact of dysfunctional DH and ER domains on polyketide size and shape. A) Module skipping does not occur in DH2 null and ER2 null strains. Traces of 2 formed in all mutants due incomplete ketoreduction in AurB. B) HPLC profiles of (i) aureothin reference 1, (ii) luteoreticulin reference 2, (iii) metabolites of S. albus::pHJ48 (aurPKS), (iv) pHY127 (KR2 null mutant), (v) pHY140 (DH2 null mutant), (vi) pHY147 (ER2 null mutant). UV detection is at 350 nm. C) Recombinant AurH converts compound 3 to 4 and 5 in vitro. Extracted ion chromatogram of (i) references of 3, 4, and 5, (ii) control experiment with heat‐inactivated AurH, (iii) biotransformation of 3 with AurH.