Figure 4.

Weekly alternating light‐dark cycles increase gene expression of markers of inflammation, oxidative stress, and leukocyte recruitment within the aortic vessel wall. APOE*3‐Leiden.CETP mice were exposed to either regular light‐dark cycles (LD) or weekly alternating light‐dark cycles (12 h shifts; LD‐DL) (n = 18/group) for 10 wk, after which they were sacrificed at either ZT0 or ZT12 (n = 9 per timepoint/group). Aortas were isolated, and gene expression of markers of (A‐D) inflammation, (E‐L) oxidative stress, and (M‐P) leukocyte recruitment was measured by qRT‐PCR. Data represent means ± SEM. *P < .05, **P < .01, and ***P < .001 compared with the indicated control group, according to two‐way ANOVA