Fig. 2.

Effects of Cl-EE on cell viability and NO production in LPS-activated RAW 264.7 macrophages. a Effect of Cl-EE on the cell viability. RAW264.7 cells were treated with various concentrations of Cl-EE for 24 h and the cell viability was measured by MTT assay. b Effect of Cl-EE on LPS-induced NO production. RAW264.7 cells were pretreated with Cl-EE at the indicated concentrations for 1 h and then exposed to LPS (10 ng/mL) for 24 h. The nitrite levels in the medium were measured by the Griess reaction. ^##P < 0.01 versus normal control (NC) group; ^**P < 0.01 versus LPS alone