Skip to main content
. 2019 Dec 17;10:392. doi: 10.1186/s13287-019-1499-z

Table 1.

Sequences of primers used in qPCR

Gene Primer Sequence 5′–3′ Amplicon length (bp) Accession no.
Vimentin F: GCAGGATTTCTCTGCCTCTT 203 XM_014846038.1
R: TATTGCTGCACCAAGTGTGT
Decorin F: GATGCAGCTAGCCTGAGAGG 248 XM 014841263.1
R: GTGTTGTATCCAGGTGGGCA
Col2A1 F: ATTCCTGGAGCCAAAGGATCTGCT 148 XM_014850051.1
R: TGAAGCCAGCAATACCAGGTTCAC
ACAN F: TGGTGTCCTCTTCTTGTCGCTTTC 160 XM_014733894.1
R: ACGATACATTTGCTGTGCTTCGGC
COMP F: AGTGTCGCAAGGATAACTGCGTGA 238 NM_001081856.1
R: TCCTGATCTGTGTCCTTCTGGTCA
SOX-9 F: GAACGCCTTCATGGTGTGGG 225 XM_014736619.1
R: TTCTTCACCGACTTCCTCCG
Runx-2 F: CCAAGTGGCAAGGTTCAACG 165 XM_014837484.1
R: TGTCTGTGCCTTCTGGGTTC
ALP F: AGGCCAAGATGAGGGTAGCA 352 XM_014852743.1
R: TCTCTGGCACTAAGGAGTTGGT
VEGF F: CCCACTGCGGAGTTCAACAT 167 XM_014837457.1
R: TTTCTCCGCTCTGAGCAAGG
CD44 F: CCTCCAGCGAAAGAAGCACT 137 NM_001085435.2
R: GGCTCGGTCTTTGGTAGTGG
p53 F: TACTCCCCTGCCCTCAACAA 252 U37120.1
R: AGGAATCAGGGCCTTGAGGA
Bax F: TTCCGACGGCAACTTCAACT 204 XM_005596728.1
R: GGTGACCCAAAGTCGGAGAG
Bcl-2 F: TTCTTTGAGTTCGGTGGGGT 164 XM_014843802.1
R: GGGCCGTACAGTTCCACAA
p21 F: GAAGAGAAACCCCCAGCTCC 241 XM_003365840.2
R: TGACTGCATCAAACCCCACA
Sod1 (Cu/Zn SOD) F: CATTCCATCATTGGCCGCAC 130 NW_001867397.1
R: GAGCGATCCCAATCACACCA
Sod2 (Mn SOD) F: GGACAAACCTGAGCCCCAAT 125 NW_001867408.1
R: TTGGACACCAGCCGATACAG
CAT F: ACCAAGGTTTGGCCTCACAA 112 XM_014851065.1
R: TTGGGTCAAAGGCCAACTGT
GAPDH F: GATGCCCCAATGTTTGTGA 250 NM_001163856.1
R: AAGCAGGGATGATGTTCTGG

Sequences: amplicon length and access numbers of the primer sets. Cal2A1 Collagen type II alpha 1 chain, ACAN Aggrecan, COMP cartilage oligomeric matrix protein, Sox-9 transcription factor SOX-9, Runx-2 Runt-related transcription factor 2, ALP alkaline phosphatase, VEGF vascular endothelial growth factor, CD44 Homing cell adhesion molecule, p21 cyclin dependent kinase inhibitor 1A, p53 tumor suppressor p53, BCL-2 B-cell lymphoma 2, BAX BCL-2-associated X protein, Sod1 (Cu/Zn SOD) copper-zinc-dependant superoxide dismutase, (CuZnSOD; Sod2 (Mn SOD) manganese-dependent superoxide dismutase (MnSOD), GADPH glyceraldehyde-3-phosphate dehydrogenase