Figure 2.
GAIN domain cleavage is required for GPR56 activation. A, cells transfected with WT GPR56 and GPR56H381S were treated with 3-α-DOG (5 μm), P19 (20 μm), or collagen III (Col III, 50 nm) in an SRE–luciferase reporter assay. Data are normalized to the Renilla luciferase control and expressed as -fold over the signal obtained from cells transfected with SRE–luciferase only. Data are presented as mean ± S.D.; n = 3; *, p < 0.05; ***, p < 0.001; ****, p < 0.0001; two-tailed Student's t test. B and C, RhoA activation by treatment with 3-α-DOG (5 μm), P19 (20 μm), or collagen III (50 nm) on WT GPR56-expressing HEK293T cells. Data are presented as mean ± S.D.; n = 3; *, p < 0.05; **, p < 0.01; two-tailed Student's t test.