Figure 3.
TG2 requires the presence of the ECM protein laminin to activate GPR56. A, TG2 promotes oligodendrocyte precursor cell proliferation in an GPR56- and laminin (Lam)-dependent manner. B, cells transfected with WT GPR56 and GPR56H381S were treated with TG2 (200 nm), laminin (2 μg/ml), or TG2 plus laminin in an SRE–luciferase reporter assay. Data are normalized to Renilla luciferase control and expressed as -fold over the signal obtained from cells transfected with SRE–luciferase only. Data are presented as mean ± S.D.; n = 3; **, p < 0.01; ns, not significant; two-tailed Student's t test. C and D, RhoA activation by treatment with TG2 (200 nm) or TG2 plus laminin (2 μg/ml) of WT GPR56-expressing HEK293T cells. Data are presented as mean ± S.D.; n = 3; **, p < 0.01; two-tailed Student's t test. Fib, fibronectin.