Table 5.

Tau-mediated polymerization

Polymerization t_½ for Tau constructs shown in Figs. 5 and 6. Values listed for t_½ are mean ± S.D. for n = 3 independent measurements. NA indicates constructs tested that did not measurably polymerize under assay conditions. The tubulin concentration was held constant at 10 μm across all polymerization experiments, with the Tau concentration varied according to the table below. Polymerizations were carried out in phosphate buffer (pH 6.9) with 1 mm GTP at 37 °C.

Construct	t½ (s)	[Tau] (μm)
PRR-MTBR-R'	52 ± 7	5
tau2N	137 ± 9	5
tau1N	85 ± 5	5
tau1N*	88 ± 13	5
tau0N	76 ± 10	5
PRR	96 ± 55	10
2N-PRR	NA	10
MTBR	NA	10
MTBR-R'	NA	10
2N-MTBR-R'	NA	10