Figure 3.

Rrp6 remains associated with the core exosome complex and is retained in immature pre-ribosomal subunits upon depletion of Nop53. A, proteins that showed a statistically significant enrichment or depletion (both orange and blue dots in Fig. 4B) in the absence of Nop53 were categorized according to their biological function in a pie chart. Among the most enriched classes, both ribosome biogenesis and exosome complex stood out. B, same proteins, significantly affected by Nop53 depletion, are depicted with their respective fold changes. Positive and negative fold change values indicate, respectively, enrichment and decrease upon Nop53 depletion. Of note, several early pre-60S and pre-40S transacting factors were found coimmunoprecipitated with increased levels in the absence of Nop53. The exosome subunits and cofactors were slightly enriched, but not as much as the RNA helicase Mtr4.