Figure 6.

PpiD also interacts with YidC. A, in vivo p-formaldehyde (PFA) cross-linking was performed with BL21 cells expressing YidC. After PFA treatment, YidC was purified via metal-affinity chromatography, separated by SDS-PAGE, and analyzed by Western blotting with α-PpiD antibodies. PpiD and its potential cross-link to YidC are indicated. B, in vivo photocross-linking was performed with BL21 cells expressing either YidC WT or YidC with pBpa incorporated at position 399 within the cytosolic loop C1 of YidC. YidC was purified via metal-affinity chromatography and analyzed by SDS-PAGE and Western blotting with α-PpiD (top) or α-YidC (bottom) antibodies.